Competitive partial inhibitors of serum albumin‐catalyzed sulfur cyanolysis
作者:
Rebecca Jarabak,
John Westley,
期刊:
Journal of Biochemical Toxicology
(WILEY Available online 1990)
卷期:
Volume 5,
issue 1
页码: 1-8
ISSN:0887-2082
年代: 1990
DOI:10.1002/jbt.2570050102
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Serum Albumin;Sulfur Cyanolysis;Competitive Partial Inhibition;Short‐Chain Fatty Acids;Dansyl Compounds;Sulfur‐Binding Sites
数据来源: WILEY
摘要:
AbstractEfforts to locate the active site for sulfur cyanolysis catalyzed by bovine serum albumin have led to systematic tests of several compounds that inhibit the catalyzed reaction. Hexanoate and 5‐dimethylaminonaphthalene‐1‐sulfonate bind at the same site and are partial inhibitors competitive with cyanide, uncompetitive with respect to sulfur. Various dansyl amino acids and 1‐anilino‐8‐naphthalene sulfonate display the same inhibitory behavior but bis (1‐anilino‐8‐naphthalene sulfonate) is a total inhibitor competitive with cyanide. These findings are interpreted to indicate that the cyanolysis active site is near, but not at, one of the short‐chain fatty acid binding sites on albumin subdomain 2‐AB or 3‐AB. Both ionic repulsion and steric considerations are implicated in the m
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