Human Brain Capillary Endothelium2-Arachidonoglycerol (Endocannabinoid) Interacts With Endothelin-1
作者:
Ye Chen,
Richard McCarron,
Yukoh Ohara,
Joliet Bembry,
Nabil Azzam,
Fred Lenz,
Esther Shohami,
Raphael Mechoulam,
Maria Spatz,
期刊:
Circulation Research: Journal of the American Heart Association
(OVID Available online 2000)
卷期:
Volume 87,
issue 4
页码: 323-327
ISSN:0009-7330
年代: 2000
出版商: OVID
关键词: brain endothelial function;2-arachidonoglycerol;endothelin-1;endothelium;cannabinoids
数据来源: OVID
摘要:
In brain, the regulatory mechanism of the endothelial reactivity to nitric oxide and endothelin-1 may involve Ca2+, cytoskeleton, and vasodilator-stimulated phosphoprotein changes mediated by the cGMP/cGMP kinase system.1Endothelium of human brain capillaries or microvessels is used to examine the interplay of endothelin-1 with the putative vasorelaxant 2-arachidonoyl glycerol, an endogenous cannabimimetic derivative of arachidonic acid. This study demonstrates that 2-arachidonoyl glycerol counteracts Ca2+mobilization and cytoskeleton rearrangement induced by endothelin-1. This event is independent of nitric oxide, cyclooxygenase, and lipoxygenase and is mediated in part by cannabimimetic CB1 receptor, G protein, phosphoinositol signal transduction pathway, and Ca2+-activated K+channels. The induced rearrangements of cellular cytoskeleton (actin or vimentin) are partly prevented by inhibition of protein kinase C or high levels of potassium chloride. The 2-arachidonoyl glycerol–induced phosphorylation of vasodilator-stimulated phosphoprotein is mediated by cAMP. These findings suggest that 2-arachidonoyl glycerol may contribute to the regulation of cerebral capillary and microvascular function.
点击下载:
PDF
(807KB)
返 回