A fast growing callus obtained after cultivating root sections of 1 sm lenght on Murashige and Skoog (MS) medium with 2 mg/l 1- naphtalene acetic acid (NAA) and 0.25 mg/l 6-benzyl- aminopurine (BAP) (pH 5.6) as the hormonal balance, was used as the source of protoplasts. Suspensions of 107 protoplasts/g of fresh weight with 94% viability were cultivated on the basic MS medium supplemented with 9% (W/v) mannitol and 2 mg/l NAA, 0.5 mg/l BAP, 0.5 mg/l zeatin (pH 5.6) for further cell wall regeneration, cell colonies formation and microcalli growth. One root regenerated after subcultivating the calli in MS medium with various combinations of NAA, BAP and Zeatin. A second root regenerated after further calli subcultures on Lepoivre medium only containing 2 mg/l indole-3-butyric acid. The paper dwells on the determinant factors for the process of rhizogenesis as an intermediate step towrds obtaining shoot buds on a cherry-tree.