Background.Recent studies have shown a rise inCandidaglabratainfections among immunocompromised adults. In published case series of neonatal candidemia, however, the speciesglabratais uncommon. We conducted a retrospective chart review to examine the epidemiology, clinical presentation and outcome of neonatal infection withC. glabratacompared with other species ofCandida.Methods.Neonatal and microbiology databases of two affiliated hospitals were searched for all cases of candidemia in neonatal intensive care unit patients with suspected sepsis from 1991 through 1998.Results.Of 58 cases ofCandidasepsis, 9 (15%) were caused byC. glabrata(CG), 41 (71%) byC. albicans(CA) and 8 (14%) byC. parapsilosis(CP). There was no change in the proportion of candidemia caused byglabrataspecies in the years studied. Although there was a significantly higher proportion of CG cases at 1 hospital (29%vs.6%,P= 0.01), there was no case clustering to suggest direct nosocomial spread. Compared with otherCandidaspecies, CG occurred in infants of higher gestational age (CG 29.7 weeks, CA 26.6 weeks, CP 27.3 weeks) and birth weight (CG 1442 g, CA 931 g, CP 965 g). Patients with CG sepsis were more likely to be receiving broad spectrum antibiotics at the time of diagnosis (CG 67%, CA 38%, CP 38%), were less likely to present with apnea and had less severe thrombocytopenia. Of 9 patients with CG sepsis, 1 had meningitis, 1 had necrotizing enterocolitis and 3 had candiduria.Conclusion.C. glabratais a significant nosocomial pathogen in the neonate.