Purpose.Microbially contaminated contact lens cases are a predisposing risk factor forAcanthamoebakeratitis. Several findings have shown that microwave irradiation kills the six Food and Drug Administration test challenge microorganisms. We aimed to determine what effect microwave irradiation has onAcanthamoebatrophozoites and cysts.Methods.Different types of contact lens cases were contaminated with trophozoites and cysts of three differentAcanthamoebaspecies (A. comandoni, A. castellanii, A. hatchetti) and were exposed to microwave irradiation for 3, 5, and 8 minutes, respectively.Results.Trophozoites, as well as cysts of the differentAcanthamoebastrains, were effectively killed, even by only 3 minutes of microwave irradiation, and there were no negative effects of irradiation on the contact lens cases themselves.Conclusion.We demonstrate that microwave treatment is a very effective, easy, and cheap method to keep contact lens cases free ofAcanthamoeba,thus considerably reducing the risk of anAcanthamoebakeratitis.