Cross‐reactivity on Western blots in HIV‐1 and HIV‐2 infections
作者:
Kevin De Cock,
Anne Porter,
Justin Kouadio,
Matthieu Maran,
Marie-France Lafontaine,
Guy-Michel Gershy-Damet,
William Heyward,
Richard Georget,
期刊:
AIDS
(OVID Available online 1991)
卷期:
Volume 5,
issue 7
页码: 859-864
ISSN:0269-9370
年代: 1991
出版商: OVID
关键词: HIV-1;HIV-2;Africa;West Africa;Western blot;synthetic peptide;serology.
数据来源: OVID
摘要:
To examine cross-reactivity of antibodies to heterologous antigens, on HIV-1 and HIV-2 Western blots, we tested sera from 1362 consecutive tuberculosis (TB) patients and 2127 consecutive blood donors. Specimens positive on enzyme-linked immunosorbent assay (ELISA) for HIV-1 or HIV-2 were further characterized by synthetic peptide-based tests, and tested by HIV-1− and HIV-2-specific Western blots. Dual serologic reactivity on synthetic peptide tests was proportionately more frequent in HIV-positive TB patients than in blood donors, and HIV-2 reactivity less frequent. Positive HIV-1 Western blots were seen in 73–83% of specimens specifically characterized as positive for HIV-2 on synthetic peptide tests. Cross-reactivity to HIV-2 Western blots by HIV-1-positive specimens was significantly more frequent in TB patients (35%) than in asymptomatic donors (9%;P< 0.001). Using recently recommended criteria for HIV-2 Western blot interpretation (presence of two env bands) reduced the overall proportion of HIV-1-positive specimens having a positive HIV-2 Western blot from 27.5 to 16.4%, suggesting minimal effect on sensitivity in the diagnosis of HIV-2 reactivity on specimens positive on synthetic peptide tests.
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