Pulse Voltammetric Biosensing System for the Rapid Determination of Glucose With Micro-Enzyme Sensor
作者:
Yoshihito Ikariyama,
Naoki Shiraada,
Shigeru Yamauchi,
Tomoaki Yukiashi,
Hiroo Ushioda,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 6
页码: 953-964
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808071923
出版商: Taylor & Francis Group
关键词: Pulse voltammetry;Platinlzation;Micro-enzyme sensor;Real time determination
数据来源: Taylor
摘要:
An electrochemically prepared micro-enzyme electrode whose diameter is 50 jim is combined with an Pt auxiliary electrode and a reference electrode to assemble a three electrode device for the rapid determination of glucose. Since the device is very small, glucose sample whose volume is only 2 μ1 can be successfully determined. Pulse voltammetry is shown to be an effective approach for making the sensing device work without any attachments such as magnetic stirrer and pump. The transient sensor output, oxidizing current for the hydrogen peroxide generated by the immobilized glucose oxidase, shows a good linearity in the glucose concentration range from 1 mM to 20 mM.
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