AbstractSterols from pollen collected by foraging honeybees,Apis melliferaL, at seven field sites were compared with the sterols of foraging adults and/or prepupae collected from colonies at each site. Invariably, the composition of prepupal sterols was comparable to that found in previous cage studies using chemically defined diets containing various dietary sterols: 24‐methyl‐enecholesterol was the major sterol; sitosterol and isofucosterol were present in lesser, but significant amounts; and a trace amount of cholesterol was identified in each sample. This occurred even though some of the pollen sterols contained little 24‐methylenecholesterol, sitosterol, or isofucosterol and a preponderance of certain other sterols, such as δ7‐stigmasten‐3β‐ol and δ7,24(28)‐campestadien‐3β‐ol in goldenrod and corn pollens, respectively. Thus the selective transfer and utilization of sterols in honeybees that have been demonstrated in cage studies with artificial diets were also shown to occur u