Development of the Fetal Mouse Palate in Suspension Organ Culture
作者:
Kohei Shiota,
Tsuneo Kosazuma,
Stephan Klug,
Diether Neubert,
期刊:
Cells Tissues Organs
(Karger Available online 1990)
卷期:
Volume 137,
issue 1
页码: 59-64
ISSN:1422-6405
年代: 1990
DOI:10.1159/000146859
出版商: S. Karger AG
关键词: Palatogenesisin vitro;Secondary palate formation;Mouse fetus;Organ culture
数据来源: Karger
摘要:
Explanted palates of day 12 and day 13 mouse fetuses were cultured in a chemically defined serumless medium for 48–72 h by a suspension culture technique. The palate of day 12 fetuses closed successfully within 72 h and that of day 13 fetuses within 48 h. Both macroscopically and histologically the in vitro fusion of palatal shelves simulated the palatogenetic process in vivo. This novel technique for culturing the fetal mouse palate may be of potential use for the study of palatogenesis and in developmental toxicolog
点击下载:
PDF
(1107KB)
返 回