Simultaneous Capillary Gas Chromatographic Determination of Cyproterone Acetate and 15β-Hydroxycyproterone Acetate in Urine
作者:
Jasmina Novakovic,
Eva Tvrzická,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 8
页码: 1657-1667
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308021487
出版商: Taylor & Francis Group
关键词: Cyproterone Acetate;15β-hydroxycyproterone Acetate;Capillary GC;Spiked Urine
数据来源: Taylor
摘要:
The simultaneous capillary GC determination of underivatized antiandrogen cyproterone acetate (CPA) and its active metabolite 15β-hydroxycyproterone acetate (OH-CPA) in spiked urine was performed on a flexible VCOT quartz capillary column, coated with a non-polar CP-Sil 5 CB liquid phase. A split/splitless injector and a flame-ionization detector were used. Equilin was used as an internal standard, and resolution of all the compounds was achieved in 6 minutes. Limit of detection was 0.04 μg/μl of injected amount for both CPA and OH-CPA, the recoveries were between 91.35% and 105.56%, and the relative standard deviation varied from 3.35% to 7.38%. The method is applicable in analysis of these steroids in biological fluids.
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