Retarded development of fetal renal alkaline phosphatase in mice given 2,4,5‐trichlorophenoxyacetic acid
作者:
B. Highman,
T. B. Gaines,
H. J. Schumacher,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1977)
卷期:
Volume 2,
issue 5
页码: 1007-1018
ISSN:0098-4108
年代: 1977
DOI:10.1080/15287397709529499
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Histologic study of the fetal offspring of maternal mice given 2,4,5‐trichlorophenoxy‐acetic acid (2,4,5‐T) suggested that the previously reported fetal “cystic kidneys” were due to a retardation in fetal renal development and downgrowth of the renal papilla into the pelvis. To determine a possible retardation in renal alkaline phosphatase or functional development, maternal mice received by gavage 60–120 mg/kg, 2,4,5‐T on days 6–14 of pregnancy. At necropsy on day 17, the fetal kidneys were excised and fixed 24 hr in cold 65% ethanol. Paraffin sections stained by Gomori's method revealed alkaline phosphatase mainly in tubules in the inner renal cortex. Fetal kidneys showing diminished or no alkaline phosphatase were designated subnormal. There was a statistically significant greater incidence of subnormal fetal kidneys in the 2,4,5‐T‐treated mice than in the untreated controls. In three experiments, some mice were also sacrificed on day 18, and the incidence of subnormal fetal kidneys was significantly lower than on day 17. This retardation in renal alkaline phosphatase development indicates a retardation in renal functional development and indirectly supports the view that 2,4,5‐T also retards the morphological development of the fetal kidney and is not a renal teratogen in mice. It also illustrates that selected histochemical studies may be helpful in a teratologic investigation.
点击下载:
PDF (761KB)
返 回