Ammonium Thiocyanate for Detaching Antibodies from Histological Specimens
作者:
IturrizaFermín C.,
Gómez DummCésar L. A.,
BianchiCésar E.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 5
页码: 239-241
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109109979
出版商: Taylor&Francis
关键词: immunohistochemistry;antibodies;peroxidase-antiperoxidase method
数据来源: Taylor
摘要:
When different antigens must be demonstrated in the same structure, the permanence of former antibodies can lead to false identification of another antigen. The peroxidase-antiperoxidase method was used, followed by the oxygen acceptor ethyl-carbazole. After staining the sections, they were destained with xylene and the antibodies detached with 3 M ammonium thiocyanate; then the specimens were treated for the demonstration of the other antigen. The procedure could be repeated and thus as many as four antigens could be demonstrated without damaging the tissues. Antigens participating in the immunohistochemical staining were well-preserved after destaining and detaching the antibodies as demonstrated by their ability to react again in a second staining.
点击下载:
PDF (682KB)
返 回