Effect of Antitubular Basement Membrane and Brush Border Antibodies onp-Aminohippurate Transport in Kidney
作者:
Richard P. Wedeen,
Vecihi Batuman,
Harold Sobel,
期刊:
Nephron
(Karger Available online 1981)
卷期:
Volume 29,
issue 5-6
页码: 258-264
ISSN:1660-8151
年代: 1981
DOI:10.1159/000182385
出版商: S. Karger AG
关键词: Antitubular antibodies;Interstitial nephritis;PAH transport
数据来源: Karger
摘要:
The effect of antiproximal tubule basement membrane (TBM) and brush border (BB) antibodies on p-aminohippurate (PAH) transport was evaluated in a rat model of immunologically mediated interstitial nephritis. Immunized rats developed anti-TBM antibody titers ranging from 1 to 3,072 with continuous linear IgG and interrupted C3 deposits in the TBM. Circulating anti-BB antibodies were detected in half of the immunized rats in titers ranging from 1 to 128. Heavy IgG deposition was present in the BB when circulating anti-BB antibody titers exceeded eight and proteinuria was present. When anti-TBM antibody titers were 1,024 or greater the slice-to-medium PAH concentration ratio (S/M) was significantly reduced (p < 0.001). Combined immunofluorescent microscopy and section freeze-dry autoradiography revealed normal cellular distribution of PAH-3H in all proximal tubules except in rare microscopic foci of interstitial nephritis in which concentrative transport was absent. However, luminal secretion of PAH-3H was strikingly reduced in tubules with heavy IgG BB deposits. 3-hour PAH excretion in vivo fell significantly in rats with circulating and tissue anti-BB antibodies. Antibody inhibition of PAH transport appeared to be independent of morphologic damage. Summary: Anti-TBM antibodies were associated with decreased slice uptake of PAH-3H while anti-BB antibodies were associated with decreased luminal PAH secretion in vitro and in vivo.
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