The Neurohormonal Thymic Microenvironment: Immunocytochemical Evidence that Thymic Nurse Cells Are Neuroendocrine Cells
作者:
Vincent Geenen,
Marie-Paule Defresne,
Françoise Robert,
Jean-Jacques Legros,
Paul Franchimont,
Jacques Boniver,
期刊:
Neuroendocrinology
(Karger Available online 1988)
卷期:
Volume 47,
issue 4
页码: 365-368
ISSN:0028-3835
年代: 1988
DOI:10.1159/000124938
出版商: S. Karger AG
关键词: Thymus;Nurse cells;Neuroendocrine markers;Frozen sections;Immunofluorescence
数据来源: Karger
摘要:
Thymic neuroendocrine cells were identified by immunofluorescence in the murine thymus through the use of monoclonal antibody A2B5, and specific polyclonal antisera against neurophysin (NP), oxytocin (OT) and arginine vasopressin (AVP). Two reactive regions were clearly identified: the subcapsular cortex and the medulla. A close correspondence was observed between A2B5-reactive and NP-immunoreactive cells in the medulla. An important epithelial population of the subcapsular cortex, the thymic nurse cells (TNCs), were found to be A2B5-positive and to contain immunoreactive NP, OT and AVP. The neuroendocrine nature of TNCs was further substantiated by their high reactivity with an antiserum against neuron-specific enolase. These observations demonstrate the presence in the thymus gland of an original neuroendocrine microenvironment which could be of functional importance in the mediation of central influences upon T lymphocyte differentiation.
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