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Biosynthese von14C-markierter Erucasäure mittels Rapspflanzen

 

作者: G. Meisgeier,   H. Eckert,   H. Müller,  

 

期刊: Isotopenpraxis Isotopes in Environmental and Health Studies  (Taylor Available online 1991)
卷期: Volume 27, issue 1  

页码: 36-38

 

ISSN:0021-1915

 

年代: 1991

 

DOI:10.1080/10256019108622460

 

出版商: Taylor & Francis Group

 

关键词: biosynthesis;labelling;biochemistry;carboxylic acids;carbon 14;labelled compounds;synthesis

 

数据来源: Taylor

 

摘要:

For the biosynthetic preparation of14C-erucic acid (C21H41COOH) by means of rape plants cv. sollux the plants were supplied with14CO2and additionally fed with14C-Sodium acetate after anthesis. After saponification of the extracted lipids the erucic acid was isolated and purified. The substance was identified by gas chromatography. The incorporation of the applied radioactivity (34 MBq14CO2; 37 MBq14C-natrium acetate) into the fatty acids amounted to 1,2 per cent. The eruric acid could be isolated from the fatty acids mixture with a specific radioactivity of 1,001 MBq/mmol and a purity of 97,2 per cent.

 

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