UV/VIS Spectrophotometric Studies on the Antileukemic Agent Glyoxalbis(Amidinohydrazone) [‘Glyoxalbis(Guanylhydrazone)’]
作者:
Mikko Koskinen,
Hannu Elo,
期刊:
Spectroscopy Letters
(Taylor Available online 1994)
卷期:
Volume 27,
issue 7
页码: 889-901
ISSN:0038-7010
年代: 1994
DOI:10.1080/00387019408006640
出版商: Taylor & Francis Group
关键词: Adenosylmethionine Decarboxylase Inhibitors;Polyamine Antimetabolites;Solvent Effects;Ultraviolet Spectroscopy;Visible Spectroscopy
数据来源: Taylor
摘要:
Glyoxal bis(amidinohydrazone) (GBG) and several analogs thereof are compounds of considerable pharmacological interest, and a variety of HPLC and MECC methods have been developed for their analysis. In these methods, detection is invariably based on the strong UV absorption of the compound. Yet, almost nothing has been known of their UV and VIS spectral properties. In the present paper the UV and VIS Spectroscopy of GBG has been studied in several solvent systems (water, 0. 03 M aqueous sodium acetate buffer, 0. 1 inM aqueous NaOH and dimethylsulfoxide). In the case of solutions in bare water, the shape of the UV spectrum depends drastically on concentration, probably because of changes in the species distribution of GBG as a function of concentration. The spectrum comprises one maximum at ca. 200 nm, and between ca. 250 nin and 400 nm an absorption region with distinctly higher absorbance. In the case of aqueous sodium acetate as well as NaOH solutions, one strong maximum can be detected (at ca. 285–288 nm and 332–337 nm, respectively). In both cases, the maximum occurs at constant wavelength, being independent of concentration. In dimethylsulfoxide, the spectrum of GBG contains an absorption band at distinctly higher wavelengths (λmax354 nm) than in any one of the aqueous solvents studied, indicating that solvent effects are considerable in the UV spectrum of GBG. In no case, distinct absorption could be detected at wavelengths higher than 400 nm. The results indicate that if aqueous media are used as elements in HPLC analyses of bis(amidinohydrazones) or as solvents in direct UV analysis, they must be buffered.
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