首页   按字顺浏览 期刊浏览 卷期浏览 Holographic methods in microscopy
Holographic methods in microscopy

 

作者: R. W. Smith,  

 

期刊: Journal of Microscopy  (WILEY Available online 1983)
卷期: Volume 129, issue 1  

页码: 29-47

 

ISSN:0022-2720

 

年代: 1983

 

DOI:10.1111/j.1365-2818.1983.tb04159.x

 

出版商: Blackwell Publishing Ltd

 

关键词: Holography;microscopy;holographic interferometry;speckle;ostracods;speckle reduction;stereomicroscopy;holographic lenses

 

数据来源: WILEY

 

摘要:

SUMMARYHolography can be used to record, on a flat photographic plate, information about a three‐dimensional object. In conventional microscopy a thin slice of an object is observed in focus and recorded. By combining microscopy and holography it is possible to encode, on the same flat record, all the depth information in a three‐dimensional microscopic object, not just a single infocus section. Any section of the three‐dimensional object may be subsequently reconstructed and brought into focus by using a suitable viewing system to decode the hologram. Arrangements for doing this are described. It is shown that in order to achieve the highest resolution imagery of a three‐dimensional object, reversed wave reconstruction is necessary.As holograms are made and reconstructed using a coherent laser light source, holographic microscopes are easily adapted for interferometry and an example of this is described. The differences between coherent and conventional imagery are briefly considered. The coherence of the illumination gives rise to the problems of coherent noise and speckle. Coherent noise is due to stray reflections in the optical system and can be reduced by using as few surfaces as possible or by using holographic lenses. A speckle reduction technique employing a new type of holographic optical element is described and its application to the stereomicroscopy of fossil ostracods con

 

点击下载:  PDF (1124KB)



返 回