Preparative HPLC. Part 2. Purification of β‐lactam derivatives using laboratory‐assembled equipment
作者:
S. A. Matlin,
L. Chan,
期刊:
Journal of High Resolution Chromatography
(WILEY Available online 1985)
卷期:
Volume 8,
issue 1
页码: 23-27
ISSN:0935-6304
年代: 1985
DOI:10.1002/jhrc.1240080106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Preparative liquid chromatography, HPLC;Aminopropyl silica bonded phases;β‐Lactams
数据来源: WILEY
摘要:
AbstractThe capabilities of a preparative chromatograph assembled at moderate cost from commercially available components are examined in relation to the purification of β‐lactam derivatives. It is shown that high efficiency separations can be achieved rapidly, economically, and on a scale sufficient to provide adequate quantities of material for spectroscopic identification and biological testing. In the synthesis of a large batch of aminopropyl silica for preparative HPLC purification of an impure sample of cephacetrile, three different grades of silica were investigated. The material giving the best performance was prepared on a 100 g scale and baseline separations for 0.5 g loadings of the impure cephacetrile were obtained on a 30 cm × 2.2 cm i.d. column. The pure cephalosporin was recovered in high yield by freeze drying of the elu
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