Prevention of Carbon Tetrachloride-Induced Lipid Peroxidation in Liver Microsomes from Dehydroepiandrosterone-Pretreated Rats
作者:
AragnoManuela,
TamagnoElena,
PoliGiuseppe,
BoccuzziGiuseppe,
BrignardelloEnrico,
DanniOliviero,
期刊:
Free Radical Research
(Taylor Available online 1994)
卷期:
Volume 21,
issue 6
页码: 427-435
ISSN:1071-5762
年代: 1994
DOI:10.3109/10715769409056595
出版商: Taylor&Francis
关键词: Dehydroepiandrosterone;carbon tetrachloride;lipid peroxidation;covalent binding;antioxidant
数据来源: Taylor
摘要:
Dehydroepiandrosterone (DHEA), a lipid soluble steroid, administered to rats (100 mg/kg b.wt) by a single intraperitoneal injection, increases to twice its normal level in the liver microsomes. Microsomes so enriched become resistant to lipid peroxidation induced by incubation with carbon tetrachloride in the presence of a NADPH-regenerating system: also the lipid peroxidation-dependent inactivation of glucose-6-phosphatase and gamma-glutamyl transpetidase due to the haloalkane are prevented. Noteworthy, the liver microsomal drug-metabolizing enzymes and in particular the catalytic activity of cytochrome P450IIE1, responsible for the CCl4-activation, are not impaired by the supplementation with the steroid. Consistently, in DHEA-pretreated microsomes the protein covalent binding of the trichloromethyl radical (CCl3°), is similar to that of not supplemented microsomes treated with CCl4. It thus seems likely that DHEA protects liver microsomes from oxidative damage induced by carbon tetrachloride through its own antioxidant properties rather than inhibiting the metabolism of the toxin.
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