HIV‐1 infection and modulation of cytokine and growth factor expression in Kaposi's sarcoma‐derived cells in vitro
作者:
Yao Huang,
Jian Li,
Kwang-Shin Kim,
Alexander Nicolaides,
Wei Zhang,
Junming Le,
Bernard Poiesz,
Alvin Friedman-Kien,
期刊:
AIDS
(OVID Available online 1993)
卷期:
Volume 7,
issue 3
页码: 317-322
ISSN:0269-9370
年代: 1993
出版商: OVID
关键词: HIV-1;Kaposi's sarcoma;growth factors
数据来源: OVID
摘要:
ObjectivesHIV-1 transcripts have been detected in AIDS-related Kaposi's sarcoma (KS) tissues within the factor Xllla + dermal dendrocytes present in the tumor. Various cytokines and growth factors have been shown to influence the growth of KS-derived cells in vitro. HIV-1 preferentially infects CD4 + cells and has also been found to infect some CD4− cells in vitro. The susceptibility of cultured KS cells in vitro to infection with HIV-1 and the expression of interleukin (IL)-1β, IL-6 and basic fibroblast growth factor (bFGF) after exposure to HIV-1 was examined.MethodsThe susceptibility of two different KS-derived cell cultures to HIV-1 infection was examined by the expression of p24 antigen, detection of proviral sequence and electron microscopy. The expression of IL-1β, IL-6 and bFGF was detected by enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction.ResultsKS-derived cells can be infected by HIV-1 in vitro. Both KS-derived cells were found to express CD4 mRNA. The expression of IL-1β and IL-6 was increased, whereas the expression of bFGF was not stimulated after exposure of KS cells to HIV-1.ConclusionThese experiments describe the in vitro infection of KS-derived cells by HIV-1 and the expression of various cytokines and growth factor following infection. The increased production of cytokines observed following such infection may be involved in the pathogenesis of AIDS-related KS.
点击下载:
PDF
(584KB)
返 回