AbstractThe immunogold-silver technique was modified so that 1–2μm thick glycolmethacrylate sections could routinely be used for high resolution immunocytochemistry. A rabbit antihuman hemoglobin antibody was used to demonstrate the distribution of exogenous hemoglobin in mouse tissues. Inclusion of 0.1% glutaraldehyde in the primary formalin fixation improved tissue appearance. Glutaraldehyde treatment after colloidal gold antibody application increased the staining intensity, and gold chloride intensification of the silverenhanced colloidal gold further improved immunosensitivity. Thus, a more accurate analysis of hemoglobin distribution in tissue was obtained. The enhancements were also beneficial for localizing myelin basic protein in brain and for determining the proliferative status of tissue by localization of bromodeoxyuridine incorporation into nuclei.Manual capillary action staining efficiency was demonstrated during all steps in the immunogold-silver staining protocol, greatly simplifying the procedure, reducing technician time, and producing better staining consistency. The modified immunogold-silver staining technique is, thus, simple, sensitive, economical, and relatively nontoxic. (The J Histotechnol16:13, 1993)