Highly Sensitive Recording Microspectrophotometer
作者:
Britton Chance,
Robert Perry,
Lennart A˚kerman,
Bo Thorell,
期刊:
Review of Scientific Instruments
(AIP Available online 1959)
卷期:
Volume 30,
issue 8
页码: 735-741
ISSN:0034-6748
年代: 1959
DOI:10.1063/1.1716737
出版商: AIP
数据来源: AIP
摘要:
This paper describes a sensitive, automatic recording microspectrophotometer capable of measuring the light absorption of substances present at ``enzyme'' concentrations in a single living cell. Modified dynode‐feedback and measuring circuits have been especially adapted for measurement of small photocurrents at relatively high signal‐to‐noise ratios. The application of the instrument to observations of the mitochondrial aggregate (Nebenkern) of the grasshopper spermatid is discussed.
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