An Improved Sensitive Assay for Simultaneous Determination of Plasma Haloperidol and Reduced Haloperidol Levels by Liquid Chromatography Using a Coulometric Detector
作者:
M. Hariharan,
Erick Kindt,
Ted VanNoord,
Rajiv Tandon,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1989)
卷期:
Volume 11,
issue 6
页码: 701-707
ISSN:0163-4356
年代: 1989
出版商: OVID
关键词: Key WordsHaloperidol and reduced haloperidol;High-performance liquid chromatography;Coulometric detection;Plasma levels;pH influence on voltammograms
数据来源: OVID
摘要:
A simple, highly sensitive and specific high-performance liquid chromatographic method that uses a coulometric detector for the simultaneous assay of haloperidol and reduced haloperidol in human plasma has been developed, using bromoperidol as the internal standard. A reversed phase C85-mUm column (25 x 0.46 cm) and a mobile phase of phosphate buffer (pH 7.0), acetonitrile, and methanol (40:20:40 vol/vol) are used for separating the analytes. The analytes are extracted from alkalinized plasma using a mixture of pentane and isopropanol (95:5 vol/vol) and purified by back extraction into a perchloric acid solution. Teflon tubes with screw caps are used throughout the extraction work. The compounds are oxidized at a potential of + 0.90 V against a Ag/AgCl reference electrode. The detection limit of the assay is 50 ng/L using 1 mL of plasma. The average interassay coefficient of variation for samples of concentration 1–40 mUg/L is 8%. Possible drug interferences in the assay have been studied. The absolute recovery of the method is 80%. The assay has been validated by quantitating 150 schizophrenic patients' samples.
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