The absolute ofCestrum nocturnumL. (Jessamine, “Night Queen,” Solanaceae) was analyzed by GC and GC/MS. More than 130 compounds could be detected of which more than 100 were identified. The olfactorially valuable compounds that were found with a concentration higher than 1% were: linalool (3.1%), benzaldehyde (2.5%), benzyl alcohol (2.4%), phenylacetaldehyde (2.4%), cis-jasmone (2.1%), benzyl acetate (1.8%), phenol (1.6%), methyl jasmonate (1.5%), 1,8-cineole (1.4%), borneol (1.3%), eugenol (1.3%), linalyl acetate (1.2%) and citronellyl propionate (1.1%). In addition, more than 70 other interesting volatiles as well as more than 10 higher hydrocarbons (higher than C16) and more than 10 fatty acids and their esters were also identified in the absolute.