Localization of proline-like immunoreactivity in young rat retinal neurons
作者:
NishikawaShimpei,
IchiSei,
TamaiMakoto,
期刊:
Current Eye Research
(Taylor Available online 1996)
卷期:
Volume 15,
issue 9
页码: 951-957
ISSN:0271-3683
年代: 1996
DOI:10.3109/02713689609017640
出版商: Taylor&Francis
关键词: proline;immunocytochemistry;retina;antibody;horizontal cell;rat
数据来源: Taylor
摘要:
PurposeTo localize proline in the rat retina.MethodWe prepared antibodies raised against proline coupled to bovine serum albumin (BSA) with glutaraldehyde. To confirm the specificity of the antiserum, crossreactivity with other amino acids was determined using an immunodot procedure. Rat eyes were enucleated on postnatal day 21. Immunohistochemistry was performed on semi-thin sections using gold-labelled secondary antibodies and a silver-enhancement technique.ResultsImmunodots revealed that the antiserum was specific for proline. Amino acids from retinal extracts were separated on gel, transferred to BSA-glutaraldehyde treated filters, and stained with antibodies to determine if our antibody reacted only with proline. Staining was most intense on postnatal day 21 and disappeared in the adult retina. In addition to Muller cells, horizontal cells, amacrine cells, and some cells in the ganglion cell layer were labelled.ConclusionsAntibodies raised against proline revealed a transient immunoreactivity in young rat neurons. Our findings strongly indicate that the retina may express a high level of proline in the developmental stages. This result may provide a clue for clarifying the role of proline in the retina. Curr. Eye Res. 15: 951–957, 1996.
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