Spectrophotometric and Fluorimetric Determination of Catecholamines
作者:
FatmaBasyoni Salem,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 2
页码: 281-294
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017385
出版商: Taylor & Francis Group
关键词: Spectrophotometric;Spectrofluorometric;catecholamines;O-phenylenediamine dihydrochloride;1,2-Diaminoanthracene and 2-Aminophenol
数据来源: Taylor
摘要:
Spectrophotometric and spectrofluorometric methods have been described for determination of catecholamines in pharmaceutical formulations. These depend upon measuring the intensity of the orange colour produced on reacting catecholamines with the ethanolic solution of o-phenylenediamine dihydrochloride at 490 nm and at 560, 410 nm emission maxima with 1,2-diaminoanthrancene and 2-aminophenol. The absorbance obeys Beer's law in the concentration range of 0.092 to 2.48 mg of catechol amines spectrophotometrically from 4.5 to 240 μg spectrofluorometrically
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