The effect of ultrasound onEscherichia coliviability
作者:
D. G. Allison,
A. D'Emanuele,
P. Eginton,
A. R. Williams,
期刊:
Journal of Basic Microbiology
(WILEY Available online 1996)
卷期:
Volume 36,
issue 1
页码: 1-11
ISSN:0233-111X
年代: 1996
DOI:10.1002/jobm.3620360102
出版商: Wiley‐VCH
数据来源: WILEY
摘要:
AbstractThe effect of continuous‐wave ultrasound on the viability ofEscherichia coliHB101 was assessed using a 20 kHz ultrasonic processor. A standardised cell suspension of fixed concentration was used to investigate the influence of different physical and environmental conditions on ultrasound susceptibility. Cell viability decreased exponentially with time at different intensities of ultrasound. Increasing intensity caused a decrease in decimal reduction times. Loss of cell viability occurred primarily from the mechanical effects of ultrasound rather than free radical damage.E. colisusceptibility was also shown to vary with growth conditions, whereby cells cultivated either on agar or harvested from the stationary phase of liquid culture were significantly more susceptible to ultrasound than an equivalent population obtained from the exponential phase of liquid growth. The implication of these results is discussed in relation to the use of ultrasound as a novel means of bacterial transformatio
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