In vitroRelease of ACTH from Dispersed Rat Pars Intermedia Cells. I. Effect of Secretagogues
作者:
J. Kraicer,
A.R. Morris,
期刊:
Neuroendocrinology
(Karger Available online 1976)
卷期:
Volume 20,
issue 1
页码: 79-96
ISSN:0028-3835
年代: 1976
DOI:10.1159/000122471
出版商: S. Karger AG
关键词: Pars Intermedia;ACTH;Vasopressin;CRF;Hypothalamic extract
数据来源: Karger
摘要:
Studies were made to test the responsiveness of dispersed pars intermedia (PI) cells to a number of secretagogues that are known to alter ACTH release from the pars distalis (PD) in vitro. In summary, (a) incubation in high [K+], which will increase ACTH release from the PD, did not alter ACTH release from the PI; (b) a crude extract of rat hypothalamus (HE) increased ACTH release from PD and PI; (c) the effect of HE was not due to its vasopressin content, since pretreatment of the extract with thioglycolic acid did not modify its ACTH-releasing activity and neither lysine nor arginine vasopressin stimulated ACTH release from the PI; and (d) a partially purified CRF preparation, which will stimulate ACTH release from the PD, did not alter ACTH release from the PI. We conclude that the hypothalamus contains a substance(s) that will stimulate ACTH release from the PI and that the ‘secretagogue’ is neither vasopressin nor the same CRF that will stimulate ACTH release from the
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