Immunofluorescence localization of cystatins in human lacrimal gland and in the exorbital lacrimal gland of the rat
作者:
Masayo Takahashi,
Yoshihito Honda,
Kazuo Ogawa,
Tibor Barka,
期刊:
Acta Ophthalmologica
(WILEY Available online 1992)
卷期:
Volume 70,
issue 5
页码: 625-631
ISSN:1755-375X
年代: 1992
DOI:10.1111/j.1755-3768.1992.tb02143.x
出版商: Blackwell Publishing Ltd
关键词: lacrimal gland;cysteine proteinase inhibitor;cystatin secretion;rat;human
数据来源: WILEY
摘要:
AbstractCystatins are widely distributed natural inhibitors of cysteine proteinase. They occur both intra and extracellularly in various cells and tissue fluids including tears. Using an immunofluorescence technique with antibodies against rat cystatin S, an inhibitor of submandibular gland origin, cystatin‐like immunoreactive material was demonstrated in the acinar cells of the exorbital lacrimal gland of the rat. Administration of the cholinergic agonist carbachol caused a depletion of cystatin from the acinar cells. This depletion was followed by a partial restitution in 6–8 h. Administration of the β‐adrenergic agonist isoproterenol for 4 days, which caused a marked hypertrophy of the submandibular gland, had no effect on the structure, weight, or cystatin content of the exorbital lacrimal gland. After such treatment, however, single large cells with intense staining for cystatin were encountered. Cystatin‐like immunoreactive material was also demonstrated in human lacrimal gland using antibodies against human cystatin S. These data suggest the notion that tear cystatins are secreted by the lacrima
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