Biosensing of Hydrogen Peroxide at Carbon Paste Electrode Incorporating N-Methyl Phenazine Methosulphate, Fumed-Silica and Horseradish Peroxidase
作者:
Wanlan Luo,
Haiying Liu,
Huihong Deng,
Kang Sun,
Changhou Zhao,
Deyao Qi,
Jiaqi Deng,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 2
页码: 205-220
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708002797
出版商: Taylor & Francis Group
关键词: Biosensor;N-methyl phenazine methosulphate;Horseradish peroxidase;Hydrogen peroxide;Fumed silica;Carbon Paste Electrode
数据来源: Taylor
摘要:
Biosensing of hydrogen peroxide was made at a carbon paste electrode incorporating fumed silica, horseradish peroxidase and N-methyl phenazine methosulphate. Cyclic voltammetric and amperometric measurements were used for the first time to demonstrate the suitability of electron communication between horseradish peroxidase and a carbon paste electrode via N-methyl phenazine methosulphate, an electron transfer mediator. Performance and characteristics of the biosensor were evaluated with respect to response time, detection limit, applied potential and concentration of the mediator. Effect of applied potential and amount of the mediator in carbon paste on the Michaelis-Menten constant of the biosensor was investigated. The biosensor possessed a variety of characteristics including long stability and rapid response and high sensitivity to hydrogen peroxide, with a detection limit of 0.08 μM.
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