Enhancement of Nitrogen Dioxide-Induced Lipid Peroxidation and Dna Strand Breaking by Cysteine and Glutathione
作者:
KikugawaKiyomi,
HiramotoKazuyuki,
OkamotoYutaka,
KoYo,
期刊:
Free Radical Research
(Taylor Available online 1994)
卷期:
Volume 21,
issue 6
页码: 399-408
ISSN:1071-5762
年代: 1994
DOI:10.3109/10715769409056592
出版商: Taylor&Francis
关键词: Nitrogen dioxide;cysteine;glutathione;sulfur-containing radical;lipid peroxidation;DNA breaking
数据来源: Taylor
摘要:
Nitrogen dioxide less than 100 ppm in air induced lipid peroxidation of liposome composed of l-palmitoyl-2-arachidonylphosphatidylcholine as assessed by thiobarbituric acid reactivity. The nitrogen dioxide-induced lipid peroxidation was enhanced by cysteine, glutathione and bovine serum albumin. While the activity of nitrogen dioxide in air to induce single strand breaks of supercoiled plasmid DNA was low, the breaking was remarkably enhanced by cysteine, glutathione and bovine serum albumin. ESR spin trapping using 5,5-dimethyl-1-pyrroline N-oxide showed that certain strong oxidant(s) were generated by interaction of nitrogen dioxide and cysteine. The spin trapping using 3,5-dibromo-4-nitrosobenzene-sulfonate suggested that sulfur-containing radicals were generated by interaction of nitrogen dioxide and cysteine or glutathione. Hence, certain sulfur-containing radicals generated by the interaction which could effectively induce lipid peroxidation and DNA strand breaks.
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