Clostridial Collagenase Releases Bioactive Fragments From Extracellular Matrix Molecules
作者:
Marco,
Radice Paola,
Brun Daniela,
Bernardi Cristina,
Fontana Roberta,
Cortivo Giovanni,
期刊:
Journal of Burn Care & Rehabilitation
(OVID Available online 1999)
卷期:
Volume 20,
issue 4
页码: 282-291
ISSN:0273-8481
年代: 1999
出版商: OVID
数据来源: OVID
摘要:
The current study was designed to investigate the biological role of small extracellular matrix fragments in wound healing. Human burn eschar tissue was digested with bacterial collagenase, and small aminoacidic fragments were inoculated in both human dermal fibroblast cultures and polyvinyl alcohol sponges implanted subcutaneously in the rat. Proliferation assays on cell cultures and biochemical and histologie analyses of the animal model were then performed. Results showed that fibroblasts treated with low concentrations of eschar fragments duplicated significantly faster than controls. Biochemical and histologic data from sponge implants showed that the inflammatory response was augmented by eschar-derived fragments at postoperative day 2, whereas protein and hydroxyproline synthesis were decreased at day 14. In conclusion, these data substantiate that the application of bacterial collagenase to débride necrotic tissue may have an indirect healing effect resulting from the local release of bioactive matrix-derived fragments.
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