Cowpea Trypsin Inhibitor and Resistance to Cowpea Weevil (Coleoptera: Bruchidae) in Cowpea Variety ‘TVu 2027’
作者:
Keyan Zhu,
Joseph E. Huesing,
Richard E. Shade,
Larry L. Murdock,
期刊:
Environmental Entomology
(OUP Available online 1994)
卷期:
Volume 23,
issue 4
页码: 987-991
ISSN:0046-225X
年代: 1994
DOI:10.1093/ee/23.4.987
出版商: Oxford University Press
关键词: protease inhibitor;cowpea;Bruchid resistance in cowpea
数据来源: OUP
摘要:
Seeds of cowpea,Vigna unguiculata(L.) Walp, line ‘TVu 2027’ are moderately resistant to the cowpea weevil,Callosobruchus maculatus(F.).C. maculatuslarvae exhibit higher mortality and take longer to develop in ‘TVu 2027’ than in more susceptible seeds. Claims have been made (Gatehouse, A.M.R., J. A. Gatehouse, P. Dobie, A. M. Kilminster&D. Boulter. 1979. Biochemical basis of insect resistance inVigna unguiculata.J. Sci. Food Agric. 30: 948–958) that an elevated level of cowpea trypsin inhibitor (CPTI) confers resistance to ‘TVu 2027’. To test this hypothesis, we isolated and purified CPTI and evaluated its impact on cowpea weevil using an artificial seed system. CPTI at dietary levels up to 2.0% (wt/wt) caused no significant increase in mortality of cowpea weevils and only slight delays in within-seed developmental times. These results do not support the hypothesis that CPTI is the basis of resistance in ‘TVu 2027’.
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