The quantitative determination of glutaraldehyde, formaldehyde and acrolein in air samples, taken at hospitals, engine shops and at one garage is described. Known volumes of air were drawn through sampling tubes, containing Amberlite XAD-2, coated with 2.4-dinitrophenylhydrazine as adsorption material. The hydrazones formed were desorbed using acetonitrile as eluent. The separation of these compounds was performed on a RP C-18 column; for detection at λ = 365 ran an UV spectrofotometer was used.