SummaryWe have cloned three genes (amy1, amy2andamy3) encoding α‐amylase in the filamentous fungusAspergillus oryzae.The established overall sequences have a very high degree of homology, showing divergences mainly in the 3’‐untranslated regions. The positions and the sequences of the eight introns were found to be absolutely identical in the three genes. The sequence analysis of the 5’‐regions revealed presumptive TATA, CAAT and GC boxes. Primer extension analysis was performed to determine the transcription start. We were able to detect mRNAs fromamy1andamy3but not fromamy2with gene‐specific oligonucleotide probes complementary to the 3’‐n