Factors Affecting the Determination of β-Cyclodextrin by Phenolphthalein Spectrophotometry
作者:
Wende Li,
Junxia Zhou,
Litian Zhang,
Harold Corke,
Liansheng Yang,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 7
页码: 1201-1213
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608001469
出版商: Taylor & Francis Group
关键词: β-cyclodextrin;determination;phenolphthalein spectrophotometry;sensitivity
数据来源: Taylor
摘要:
The sensitivity of the phenolphthalein spectrophotometric method for the determination of the purity of β-cyclodextrin (β-CD) was studied under different conditions. The alkalinity of the phenolphthalein colorimetric solution and the concentration of phenolphthalein in the colorimetric solution affected the sensitivity of the spectrophotometric readings and the linear range of the calibration curve. The optimum conditions for a basic working solution at pH 10.53 were that 5 ml of phenolphthalein working solution and 5 ml of sodium borate working solution were made up to a total 50 ml of phenolphthalein colorimetric solution, and the optimum conditions for a basic working solution at pH 11.23 were that 5 ml of phenolphthalein working solution and 3 ml of sodium borate working solution were made up to 50 ml total solution. Because of the instability of the absorbance of phenolphthalein solutions, it is suggested that at over 98% purity, β-CD should be determined by other more accurate methods.
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