4′-Thio-RNA: Synthesis of Mixed Base 4′-ThioOligoribonucleotides, Nuclease Resistance, and Base Pairing Properties with Complementary Single and Double Strand
作者:
CLAUDINE LEYDIER,
LAURENT BELLON,
JEAN-LOUIS BARASCUT,
FRANçOIS MORVAN,
BERNARD RAYNER,
JEAN-LOUIS IMBACH,
期刊:
Antisense Research and Development
(MAL Available online 1995)
卷期:
Volume 5,
issue 3
页码: 167-174
ISSN:1050-5261
年代: 1995
DOI:10.1089/ard.1995.5.167
数据来源: MAL
摘要:
4′-Thio-β-D-oligoribonucleotides (12 mer and 16 mer) containing a mixed base sequence were synthesized via the phosphoramidite solid support approach. These RNA analogs showed very good nuclease resistance as compared with wild-type RNA. Furthermore, 4′-thio-β-D-oligoribonucleotides were shown to hybridize with a complementary DNA or RNA strand to form a duplex or with a DNA hairpin to form a triple helix. 4′-Thio-RNA binds more tightly to its complementary RNA strand than to its complementary DNA strand. A 4′-thio-RNA:RNA duplex is as stable as a 2′-O-methyl-RNA:RNA duplex. 4′-Thio-RNA, however, forms a 4′thio-RNA:DNA:DNA triplex with a stability similar to the corresponding triplex with al
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