Evidence for the dissociation of the hepatobiliary MRI contrast agent Mn‐DPDP
作者:
Bernard Gallez,
Goran Bacic,
Harold M. Swartz,
期刊:
Magnetic Resonance in Medicine
(WILEY Available online 1996)
卷期:
Volume 35,
issue 1
页码: 14-19
ISSN:0740-3194
年代: 1996
DOI:10.1002/mrm.1910350104
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Mn‐DPDP;EPR;31P‐NMR spectroscopy;relaxivity
数据来源: WILEY
摘要:
AbstractThese experiments assessed and quantitated the release of free manganese Mn++from the hepatobiliary contrast agent Mn‐DPDP (manganese dipyridoxal diphosphate), using several magnetic resonance techniques (EPR spectroscopy,31P‐NMR spectroscopy, and relaxometry) to differentiate between free Mn++and Mn++in complexes in various preparations. The presence of calcium and magnesium in physiological concentrations in aqueous solutions induced the release of Mn++from the complex, as did incubation of the complex in liver homogenates. After intravenous injection of 15 μmol/kg of Mn‐DPDP, both EPR and31P‐NMR spectroscopy demonstrated that Mn‐DPDP is partly dissociated (approximately 25%) in the liver. By comparingin vitroandex vivodata from the liver, we concluded that the dissociation of Mn‐DPDP occurs primarily in the liver, whereas a minor portion of the dissociated Mn found in the liver comes from dissociation of the complex in the blood. Most of the dissociated Mn in liver becomes bound to macromolecules and is responsible for the enhancement of relaxivity observed wit
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