Characterization of a Schwann cell neurite‐promoting activity that directs motoneuron axon outgrowth
作者:
L. M. Bolin,
E. M. Shooter,
期刊:
Journal of Neuroscience Research
(WILEY Available online 1994)
卷期:
Volume 37,
issue 1
页码: 23-35
ISSN:0360-4012
年代: 1994
DOI:10.1002/jnr.490370105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: rat;survival factor;neurotrophic activity;sympathetic ganglia;ciliary ganglia
数据来源: WILEY
摘要:
AbstractSchwann cells support and facilitate axonal growth during development and successful regeneration in the peripheral nerve. In the regenerating rat sciaticnerve, Schwann cells provide a trophic milieu for primary sensory, sympathetic, and motoneurons. We have characterized a neurotrophic activity produced by adult rat sciatic nerve Schwann cells and a spontaneously immortal Schwann cell clone (iSC). This activity elicits neurite outgrowth from chick embryo explants of both CNS and PNS. The iSC activity has been concentrated by cation‐exchange chromatography and compared to known neurotrophins in bioassay. Pooled bound fractions elicit neurite outgrowth from sympathetic, ciliary and motoneurons. In collagen matrix cocultures of iSC and E4 ventral horn(before motor axon extension to muscle targets), the iSC activity can direct the initial axonal extension from motoneurons. The data presented suggest that Schwann cell‐produced activity may mediate motoneuron axonal extension before contact with their peripheral source of neurotrophin. © 1994 Wiley‐Lis
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