Uptake and Distribution of Orally Applied N-Acetyl-(14C)neuraminosyl-lactose and N-Acetyl-(14C)neuraminic Acid in the Organs of Newborn Rats
作者:
W. Witt,
H. von Nicolai,
F. Zilliken,
期刊:
Annals of Nutrition and Metabolism
(Karger Available online 1979)
卷期:
Volume 23,
issue 1
页码: 51-61
ISSN:0250-6807
年代: 1979
DOI:10.1159/000176241
出版商: S. Karger AG
关键词: N-acetyl-(14C)neuraminosyl-lactose;N-acetyl-(14C)neuraminic acid;Sialyltransferase;Intestinal absorption;Carbohydrates;CMP-N-acetylneuraminic acid
数据来源: Karger
摘要:
N-acetyl-(14C)neuraminosyl-(α,2→3)lactose enzymatically prepared of CMP-NeuNAc and lactose by a particulate enzyme fraction from lactating rat mammary gland was applied orally to newborn rats and examined for uptake and distribution in relation to those of free N-acetyl-(14C)neuraminic acid. The neonates were allowed to stay with their mother before and during the incubation time up to 6 h. Within this time 70% of the given dose was excreted while 30% was retained in the body. (14C)NeuNAc-lactose activity appeared 1.5 h after application in blood, urine, and tissues and attained maximum values after 3 and 6 h, respectively. The highest uptake occurred in liver, spleen, and brain. The absorption of the trisaccharide was delayed by 30 min compared with free (14C)NeuNAc. The time courses of the curves show a slower but higher accumulation in the tissues suggesting a better utilization of the (14C)NeuNAc from (14C)NeuNAc-lactose or pecularities in the absorption of the trisaccharide by the orga
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