Modifications of some erythrocyte parameters in hibernating vertebrates, revealed by cytofluorometric techniques
作者:
S. Barni,
G. Gerzeli,
期刊:
Journal of Experimental Zoology
(WILEY Available online 1984)
卷期:
Volume 229,
issue 1
页码: 33-39
ISSN:0022-104X
年代: 1984
DOI:10.1002/jez.1402290105
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe variations of the glycoconjugate concentrations (PAS‐positive material), with special reference to the membrane sialic component have been studied during the annual cycle in erythrocytes of three mammalian species (Erinaceus europaeus, Glis glis, Talpa europaea) and of one amphibian species (Rana esculenta). InR. esculentathe structural modifications of nuclear chromatin (different degree of compactness) have also been examined through the propidium iodide intercalation at different concentrations.The membrane sialic components during hibernation increase inRana(457%),Glis(304%), andErinaceus(244%), and decrease inTalpa(59%), not really a hibernating species. These data can be related with a slowing down of erythropoiesis and with the increase of the average length of erythrocyte life in the winter. Residual glycoconjugate components are also modified with a similar, but less evident, pattern. The changes in the fluorescence fading during the annual cycle show a substantial independence from the concentration index of the PAS‐positive material; this fact might be related to molecular modifications or heterogeneity of the substrate.The DNA‐propidium iodide intercalation in frog erythrocyte chromatin decreases during the hibernation, possibly related to a reduced functional activity of
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