Profile of Chicken Macrophage Functions After Exposure to CatecholaminesIn Vitro
作者:
AliR. A.,
QureshiM. A.,
McCorkleF. M.,
期刊:
Immunopharmacology and Immunotoxicology
(Taylor Available online 1994)
卷期:
Volume 16,
issue 4
页码: 611-625
ISSN:0892-3973
年代: 1994
DOI:10.3109/08923979409019742
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractThe effects of catecholamines (CA) on various chicken macrophage functions were examined. Macrophage monolayers were exposed to. 01,. 1,. 25, 1, 2, and 5 (μg/mL of dopamine (DA), norepinephrine (NE) and epinephrine (E) for 1 hr. All CA were toxic for macrophages at 1 -5μg dose range resulting in 25-50% cell death. All CA at the. 1 and. 25μg/mL level increasedE. coliand sheep red blood cells (SRBC) phagocytosis by macrophages. the percentage of Fc-receptor positive macrophages increased after CA exposure. Prolonged exposure of macrophages (3 hr) reduced SRBC phagocytosis by DA-treated but not in NE-and E-treated macrophages. However, after 1 hr exposure and 3 hr recovery period, CA-induced changes were reversed in all but DA-treated cultures. Apomorphine and metoclopromide blocked DA whereas propranolol blocked NE and E effects suggesting specificity of the observed effects via catecholaminergic receptors on chicken macrophages. Dopamine and NE (.25μg/mL) did not affect but E exposure enhanced LPS-induced tumoricidal factor production. These findings suggest that CA modulate chicken macrophage effector functions.
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