Stimulation of Mn2+Oxidation inLeptothrix discophoraSS-1 by Cu2+and Sequence Analysis of the Region Flanking the Gene Encoding Putative Multicopper Oxidase MofA
作者:
G. J. Brouwers, P. L. A. M. Corstjens, J. P. M. de Vrind, A. Verkamman, M. de Kuyper, E. W. de Vrind-de Jong,
期刊:
Geomicrobiology Journal
(Taylor Available online 2000)
卷期:
Volume 17,
issue 1
页码: 25-33
ISSN:0149-0451
年代: 2000
DOI:10.1080/014904500270468
出版商: Informa UK Ltd
关键词: Cu2;Leptothrix Discophora;Mof Operon;Mn2 Oxidation;Multicopper Oxidase Mofa
数据来源: Taylor
摘要:
The effect of Cu2+on the Mn2+-oxidizing activity secreted byLeptothrix discophorastrain SS-1 was studied. Cu2+stimulated the activity of spent medium from stationary cultures when added during growth of the bacteria, although cell yield decreased with increasing Cu2+concentrations. The stimulation was tentatively explained by involvement of the putative multicopper oxidase MofA in Mn2+oxidation. Cu2+did not enhance the activity when added directly to spent medium, possibly because the ions had to be incorporated during synthesis of the oxidizing factor. Sequence analysis of the region downstream from themofAgene resulted in identification ofmofB, which encodes a protein with a potential peptidyl-prolyl-cis-transisomerase site, andmofC, which encodes a protein with a potential heme-binding site. The possibility thatmofA,mofB, andmofCbelong to one operon, and the possible functions of MofB and MofC, are discussed.
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