Hepatic Vascular Exclusion as a Model for Complete and Stable Hepatic Ischemia in Dogs
作者:
H. Egawa,
T. Takeuchi,
Y. Yamaoka,
Y. Taki,
T. Morimoto,
N. Yamamoto,
J. Ueda,
Y. Konishi,
R. Kagawa,
M. Washida,
K. Tanaka,
K. Ozawa,
期刊:
European Surgical Research
(Karger Available online 1991)
卷期:
Volume 23,
issue 3-4
页码: 170-178
ISSN:0014-312X
年代: 1991
DOI:10.1159/000129149
出版商: S. Karger AG
关键词: Centripetal pump-driven active bypass;Portal decompression;Arterial ketone body ratio;Liver ischemia;Total vascular exclusion
数据来源: Karger
摘要:
In order to confirm a complete ischemia model, 1-hour warm hepatic ischemia by hepatic vascular exclusion (HVE) was studied in dogs, in comparison with that by inflow occlusion (IOC) only. The splanchnic venous bed and/or infrahepatic inferior vena cava were decompressed by a centripetal pump-driven venovenous bypass. Indocyanine green retention test revealed no hepatic blood flow in the HVE model during ischemia, while hepatic blood perfusion was still present in the IOC model. All 5 of the IOC dogs survived more than 7 days after revascularization, while 4 of the 5 HVE dogs died within 9 h. After the induction of hepatic ischemia, lactate increased in both HVE and IOC dogs. After revascularization, transaminases and guanase were elevated, the arterial ketone body ratio (acetoacetate/3-hydroxybutyrate) decreased and the serum lactate accumulated more in HVE dogs than in IOC dogs. The hepatic redox state of IOC dogs was significantly decreased by additional clamping of the inferior vena cava. It is concluded that the HVE model with a pump-driven active bypass provides complete and stable hepatic ischemia, resulting in greater deterioration of hepatic cellular functions; hence it is more suitable as a model of complete hepatic ischemia than the IOC one.
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