The large‐scale iron enrichment conducted in the NE Pacific during the Subarctic Ecosystem Response to Iron Enrichment Study (SERIES) triggered a phytoplankton bloom dominated successively by nanophytoplankton and large diatoms. During the first 14 days, surface dimethyl sulfide (DMS) levels increased both inside (up to 22 nmol L−1) and outside (up to 19 nmol L−1) the patch, with no consistent Fe effect. Later, DMS concentrations became sixfold lower inside the patch than outside. In this study, we used a DMS budget module embedded in a one‐dimensional ocean turbulence model to investigate the contribution of the interacting physical, photochemical, and biological processes to this particular DMS response. Temporal variations in biological net DMS production were reconstructed using an inverse modeling approach. Our results show that short‐term (days) variations in both the physical processes (i.e., turbulent mixing and ventilation) and the biological cycling of DMS are needed to explain the time evolution of DMS concentrations both outside and inside the Fe‐enriched patch. The biological net DMS production was generally high (up to 0.35 nmol L−1h−1) and comparable outside and inside the patch during the first 10 days, corresponding to the observed accumulation of DMS inside and outside the patch. Later, it became negative (net DMS biological consumption) inside the patch, suggesting a change in dimethylsulfoniopropionate bacterial metabolism. This study stresses the importance of short‐term variations in biological processes and their sensitivity to the physical environment in shaping the DMS response t