Device to Prepare Extruded Nuclei and Chromosome Squashes
作者:
BerriosMiguel,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 2
页码: 78-80
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106265
出版商: Taylor&Francis
关键词: Drosophila;nucleus;chromosomes;cytochemistry;immunocytochemistry;in situ hybridization;microscopy
数据来源: Taylor
摘要:
A horizontal toggle clamp mounted on a rigid base plate makes nuclear extrusion and polytene chromosome squashing simple and reproducible. The base plate has a stage with shallow flat grooves to align the tissue sample directly below the clamp's swivel foot and hold the microscope slide in place during squashing. Appropriate pressure to obtain either extruded nuclei or squash preparations of polytene chromosomes is established empirically by adjusting the clamp's spindle assembly up or down.
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