Inhibition of proliferative responses of mouse spleen lymphocytes by bovine milk κ‐casein digests
作者:
Hajime Otani,
Makoto Monnai,
期刊:
Food and Agricultural Immunology
(Taylor Available online 1993)
卷期:
Volume 5,
issue 4
页码: 219-229
ISSN:0954-0105
年代: 1993
DOI:10.1080/09540109309354801
出版商: Taylor & Francis Group
关键词: Bovine κ‐casein;lymphocytes;inhibition of proliferation;κ‐caseinoglycopeptide;neuraminidase digestion;proteinase digestion
数据来源: Taylor
摘要:
The inhibitory effects of bovine milk κ‐casein and its enzymatic digests on the proliferative responses of mouse spleen lymphocytes induced or not induced by mitogens were studied with a colorimetric assay using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT). κ‐Casein and its glyco‐macropeptide (residues 106–169) inhibited the lipopolysaccharide (LPS)‐induced proliferative response, but the inhibitory effect was lost significantly after neuraminidase and chymotrypsin digestions. In contrast, trypsin and pronase digestions of K‐casein increased inhibitory effects. The pronase digest inhibited the proliferative responses not only induced by LPS but also in the absence of mitogen or when induced by concanavalin A and phytohemagglutinin. The pronase digest seemed to possess weak cytotoxity for lymphocytes. The inhibitory peptide was a glycopeptide(s) having specific size, and the inhibitory effects were reduced significantly by neuraminidase and chymotrypsin digestions. Moreover, similar inhibitory effects on proliferation of lymphocytes were observed in pronase‐digested bovine milk αs1‐casein and β‐casein. These findings suggest that some peptides produced from milk caseins by the action of gastrointestinal proteinases might contribute to down‐regulate the immune response of neonates.
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