TEMPERATURE-SENSITIVE LATEX PARTICLES FOR IMMOBILIZATION OF α-AMYLASE
作者:
Yi-Ming Sun,
Chun-Wen Yu,
Huang-Chien Liang,
Jyh-Ping Chen,
期刊:
Journal of Dispersion Science and Technology
(Taylor Available online 1999)
卷期:
Volume 20,
issue 3
页码: 907-920
ISSN:0193-2691
年代: 1999
DOI:10.1080/01932699908943827
出版商: Taylor & Francis Group
关键词: poly(N-isopropylacrylamide);α-amylase;temperature-sensitive;enzyme immobilization;emulsion polymerization.
数据来源: Taylor
摘要:
Poly(methyl methacrylate-co-N-isopropylacrylamide-co-N-acryloxysuccin-imide) (poly(MMA/NIPAAni/NAS)) latex particles were prepared by emulsifier-free emulsion polymerization. The latex particles could reversibly flocculate and disperse when the solution temperature was kept above and below the characteristic lower critical solution temperature (LCST). The LCST of the latex dispersion could be adjusted lower or higher by increasing or decreasing, respectively, the ionic strength of the solution.α-Amylase was immobilized onto the latex particles by reacting with the succinimide groups on NAS. It was demonstrated that the ct-amylase immobilized latex particles could be separated by thermoflocculation. After 5 repeated thermocycles, 83 % of the latex particles could be recovered, and the apparent enzymatic activity could be retained as high as 78percnt;.
点击下载:
PDF (282KB)
返 回