Acid Ethanol Fixation and Polyester Wax Embedding Combines Preservation of Antigenic Determinants with Good Morphology and Enables Simultaneous Bromodeoxyuridine (BRDU) Labeling
作者:
RohollP. J. M.,
DullensH. F. J.,
KleijneJ.,
DubbinkE. J.,
OtterW. Den,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 2
页码: 55-62
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110551
出版商: Taylor&Francis
关键词: polyester wax embedding;double staining
数据来源: Taylor
摘要:
Lymphoid tissue, and/or isolated peripheral mononuclear blood cells were fixed in acid ethanol and embedded in polyester wax (melting point 37 C). The excellent cytomorphol-ogy obtained allowed distinguishing different types of individual lymphoid and nonlymphoid cells. Furthermore, this procedure was satisfactory in the immunophenotyping of histiocytes, endothelial, mesenchymal, epithelial cells, different (sub-) types of lymphocytes and also of lym-phokine activated killer (LAK) cells. The staining patterns obtained with the different poly- and monoclonal antibodies on polyester wax sections were not only analogous to those obtained on frozen sections, but cells which had incorporated bromodeoxyuridine could be double labeled with specific antiserum.
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