Studies on Synthesis and Chromatographic Properties of NAD Covalently Bound onto Phospholipid-Coated Aminated Silica
作者:
Jinmao You,
Guohuang Xie,
Changli Zhou,
Jixin Su,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 15
页码: 2705-2721
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708001815
出版商: Taylor & Francis Group
关键词: Nicotinamide adenine dinucleotide (NAD);affinity chromatography;nucleoside;nucleotide;HPLC
数据来源: Taylor
摘要:
Recently affinity chromatography with a biospecific stationary phase was widely used in separation and purification processes of all kinds of enzymes. In this paper, a series of synthetic reactions of solid-liquid phase on a silica surface are described. By using a wide-pore (30μm). microspheric silica (8μm) as the matrix and γ-aminopropyltriethoxy-silane as the activating agent, the nicotinamide adenine dinucleotide (NAD) was bonded through its amino groups with carboxylic groups of linked phospholipid by a covalent bond on the aminated supports. This bonded stationary phase provided high thermal stability which could be used for separating nucleotides with good resolution. At the same time, the effects of pH, organic modifier and ionic strength on the retention properties of nucleotides were also investigated.
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